Northwestern University Feinberg School of Medicine
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Metabolomics Developing Core Facility

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The mission of the developing Metabolomics Core Facility at Robert H. Lurie Comprehensive Cancer Center of Northwestern University is to provide LC-MS based metabolomics service including identification, semi-quantification and quantification of primary and secondary metabolites from biological samples such as cultured cells, tissues and body fluids.  The Metabolomics Core supports investigators engaged in basic, preclinical and clinical cancer research, including scientists examining basic mechanisms in of disease, as well as those seeking to identify novel targets for therapy or biomarkers that can be used for early detection, diagnosis, prognosis or response to therapy.

Contact Us

Faculty Director

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Navdeep Chandel, PhD 
Professor of Medicine
(Pulmonary and Critical Care) and Cellular Biology

McGaw Pavilion Suite M-300
Northwestern University
240 E Huron
Chicago, IL, 60611

Operations Director

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Peng Gao, PhD
Research Assistant Professor of Medicine (Hematology and Oncology)

Olson Pavilion 8-310
Northwestern University
710 N Fairbanks Ct
Chicago, IL 60611

Locations

Olson, 8-310
710 N. Fairbanks Court
Chicago, IL 60611

 

Phone

  • 312-908-8312

Services & Equipment

 Key Services

  • Metabolites Profiling - Comprehensive Hydrophilic Panel - Sample Run
    Use LCMS to analyze sample extractions for a broad spectrum of water soluble metabolites (measure 150 plus common metabolites including amino acids, nucleotides and nucleobase/nucleosides species, TCA cycle, glycolysis, pyridine and cofactor metabolites, TCA cycle metabolites,  2-HG, a-KG, pentose phosphate pathway, etc.), 30-40 minute LC-MS/MS run, raw data will be provided.  Charged by per injection.
  • Metablites Profiling - Targeted Species - Sample Run
    Use LCMS to analyze sample extractions for special water solubale metabolites on a particular pathway, 30-40 minute LC-MS/MS run, raw data will be provided. Charged by per injection.
  • Metablites Profiling – Fluxomics - Sample Run
    Use stable isotopes, such as 13C, 15N, 2H to study metabolic flux in different pathways simultaneously, 40 minute LC-MS/MS run, raw data will be provided. Charged by per injection.
  • Metabolites Quantification - Sample Run
    Use LCMS to quantify metabolites targets from sample extractions, 30-40 minute LC-MS/MS run, raw data will be provided. Charged by per injection.
  • Method Development
    Develop new assay per customer request. Charged by time (per hour).
  • Sample Preparation
    Process samples for LCMS injection. Charged by time (per hour).
  • Data analysis
    Use sofeware to analyze LCMS data to acuire peak area of metabolites, then generate report. Charged by time (per hour).
  • Consulting
    Add-on charge for consultation effort above and beyond what is included in services 1-7. Charged by time (per hour).

 Equipment

  • UPLC-MS (high accuracy and high definition): Thermo Ultimate-3000 + Q-exactive
  • Speed-Vac

 Sample Submission Process & More Information

  • Sample Submission Form
    1. Contact core to discuss project about targets, sample preparation and analysis
    2. Submit samples together with sample submission form
    3. Contact core to confirm sample reception and projected date for result returning
  • Target List (please contact us)
  • Price List (please contact us)

 Highlighted Projects

 Acknowledgement

Work performed in Metabolomics Core Facility should be acknowledged as following:

Metabolomics services were performed by the Metabolomics Core Facility at Robert H. Lurie Comprehensive Cancer Center of Northwestern University.

 

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