Northwestern University Feinberg School of Medicine
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High Throughput Analysis Laboratory

High Throughput Analysis Lab

The High Throughput Analysis Laboratory (HTA) provides researchers with equipment and expertise for the development and execution of high throughput biological analysis and screening. The facility is equipped with state-of-the-art liquid handling, plate detection and automated microbial culture handling capabilities. In addition, the HTA also is a resource for biological and chemical libraries for RNAi and other screening projects, housing two advanced whole-genome shRNAmir libraries (mouse and human) from Open Biosystems, seven yeast libraries for various knock-out or over-expression experiments and 60,000 chemical compounds from ChemDiv, ChemBridge, ASDI and NCI. The facility also develops novel automated methods to increase assay and sample preparation throughput.

 

Contact Us

Locations

  • Hogan Hall, Evanston Campus
    2205 Tech Drive, Fourth Floor
    Evanston, IL 60208
  • Silverman Hall, Evanston Campus
    2170 Campus Dr.
    Evanston, IL 60208

Phone

  • 847-491-5960

Website

Services & Equipment

 Key Services

NU-HTA provides researchers access to cutting edge technology for large scale screening and drug discovery.  Levels of service include the full range from walk-up instrument use to fully supported projects.

Selected services include:

  • Macromolecular binding, biochemical, and cell-based assays
  • High content screening with widefield or confocal optics
  • Nanoliter liquid handling up to 1536-well density
  • Whole-plate kinetic assays (ion currents, GPCR signaling)
  • Compound library screening
  • CRISPR/Cas9 screening (multiplexed libraries)
  • Analysis of large data sets
  • Fluorescence Thermal Shift assay (measures protein melting)
  • Complex liquid handling work flows

 Equipment

Analysis

  • Perkin Elmer Enspire Multimode Plate Reader
  • Molecular Devices ImageXpress High Content Imager
  • BioRad IQ5 Real-Time PCR Detection System
  • BioRad CFX384 Real-Time PCR Detection System
  • Molecular Devices FLIPRtetra
  • Biotek Synergy 4 Microplate Reader
  • Tecan Infinite M1000 Microplate Reader

Liquid Handling

  • Echo 550, Acoustic Liquid Transfer System
  • Tecan Fluent Liquid Handling system
  • Formulatrix Mantis Liquid Dispenser
  • TTP Labtech Mosquito Crystal
  • Integra ViaFILL Automated Dispenser
  • Tecan HydroSpeed Plate Washers

 Acknowledgement

All manuscripts and grants presenting work supported by this core should include the following acknowledgement:

"This work was supported by the NorthwesternUniversity High Throughput Analysis Laboratory."

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